Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. 23017) can be used to mount skeletal muscle sections stained with bungarotoxin.Ĭy Dye is a registered trademark of Cytiva. We have, however, found that EverBrite TrueBlack® Mounting Medium (cat. While we would usually recommend our TrueBlack® lipofuscin quenchers for human brain tissue, they are not compatible with bungarotoxin staining. This usually shows up as bright, punctate dots around cell nuclei. However, if you are staining human tissue (especially brain), lipofuscin autofluorescence may be bright in all channels. While the tissue shows autofluorescence, the bungarotoxin staining of motor endplates is usually much brighter than the background for all of the dye colors we’ve tested. We test fluorescent bungarotoxin on rat skeletal muscle sections. 00009) is a far-red conjugate for the Cy®5 channel with a low negative charge that should have low background from either the dye or autofluorescence. Endogenous fluorescence background in tissue is usually highest in the blue wavelengths (DAPI channel) and lowest in the far-red (Cy®5 channel). When staining tissues, the endogenous autofluorescence of the tissue itself is often the most significant source of background. Usually, this is more of a concern with labeled antibodies that carry many dyes, as opposed to a small toxin like bungarotoxin. Opal is a trademark of Akoya Biosciences.ĭyes that carry multiple negative charges can introduce background. Note: The CF® Dyes listed here are spectrally similar alternatives to Opal™ dyes, they have not been validated as direct replacements for use with Akoya's kits or imaging system. CF® Dye Alternatives for Akoya Tyramides Akoya Tyramide They have not been validated as direct replacements for use with Akoya kits or imaging systems. Unless noted as a direct replacement, these tyramide dyes are spectrally similar alternatives. However, a better choice for multiple labeling is Rhodamine Red-X because its fluorescence is midway between a green fluorescing dye (like Alexa Fluor 488) and a far-red-fluorescing dye like Alexa Fluor 647.See below for our recommended alternatives to Akoya tyramides. C圓 can also be paired with Alexa Fluor 647 for multiple labeling when using a confocal microscope. C圓 has been used with fluorescein for double labeling however, the use of a narrow band-pass emission filter for fluorescein is recommended to minimize C圓 fluorescence in the FITC filter set. C圓 can be excited to about 50% of maximum with an argon laser (514 nm or 528 nm lines), or to about 75% of maximum with a helium/neon laser (543 nm line) or mercury lamp (546 nm line). We recommend C圓 as a brighter alternative to TRITC. For fluorescence microscopy, C圓 can be visualized with traditional tetramethyl rhodamine (TRITC) filter sets, since the excitation and emission spectra are nearly identical to those of TRITC. C圓 conjugates can be excited maximally at 550 nm, with peak emission at 570 nm. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins, but it may cross-react with immunoglobulins from other species.Ĭ圓 is brighter, more photostable, and gives less background than other orange-red fluorescing dye conjugates. No antibody was detected against non immunoglobulin serum proteins. It also reacts with the light chains of other chicken immunoglobulins. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule chicken IgY.
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